Comparison of two reducing agents dithiothreitol and tris(3-hydroxypropyl)phosphine for in vitro kinetic assay of vitamin K epoxide reductase
| dc.contributor.author | Takeda, Kazuki | |
| dc.contributor.author | Ikenaka, Yoshinori | |
| dc.contributor.author | Morita, Ayuka | |
| dc.contributor.author | Nakayama, Shouta M.M. | |
| dc.contributor.author | Ishizuka, Mayumi | |
| dc.contributor.researchID | 27878368 - Ikenaka, Yoshinori | |
| dc.date.accessioned | 2020-03-19T08:15:11Z | |
| dc.date.available | 2020-03-19T08:15:11Z | |
| dc.date.issued | 2020 | |
| dc.description.abstract | Vitamin K epoxide reductase (VKOR) is a target enzyme for anticoagulants, such as warfarin, that are used as medicines or rodenticides. Assessing VKOR activity is required to ensure the proper usage of these drugs. Dithiothreitol (DTT) is a typical disulfide reductant that is used as a substrate for in vitro VKOR assays. However, DTT is considered problematic because of its side effects. Tris(3-hydroxypropyl)phosphine (THP) has been found to be a reliable alternative to DTT, as shown by kinetic analyses of the VKOR with them. THP showed significantly lower Vmax and Km values than those of DTT; however, there was no significant difference in their Vmax/Km and IC50 for warfarin | en_US |
| dc.identifier.citation | Takeda, K. et al. 2020. Comparison of two reducing agents dithiothreitol and tris(3-hydroxypropyl)phosphine for in vitro kinetic assay of vitamin K epoxide reductase. Veterinary and animal science, 9: #100095. [https://doi.org/10.1016/j.vas.2020.100095] | en_US |
| dc.identifier.issn | 2451-943X | |
| dc.identifier.uri | http://hdl.handle.net/10394/34418 | |
| dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S2451943X20300089 | |
| dc.identifier.uri | https://doi.org/10.1016/j.vas.2020.100095 | |
| dc.language.iso | en | en_US |
| dc.publisher | Elsevier | en_US |
| dc.subject | Vitamin K | en_US |
| dc.subject | Vitamin K epoxide reductase | en_US |
| dc.subject | Dithiothreitol | en_US |
| dc.subject | Tris(3-hydroxypropyl)phosphine | en_US |
| dc.subject | Anticoagulant | en_US |
| dc.title | Comparison of two reducing agents dithiothreitol and tris(3-hydroxypropyl)phosphine for in vitro kinetic assay of vitamin K epoxide reductase | en_US |
| dc.type | Article | en_US |