A quantitative assessment of cell-free DNA utilizing several housekeeping genes: measurements from four different cell lines
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Aucamp, Janine
Bronkhorst, Abel Jacobus
Wentzel, Johannes F.
Pretorius, Piet J.
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Springer
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Abstract
Quantitative real-time PCR (qPCR) is regularly used to quantify cell-free nucleic acids (cfNAs) in order to identify biomarkers for various pathologies. However, studies have shown notable housekeeping gene expression variation between healthy and diseased tissues and treated versus untreated cell lines. The release of housekeeping genes by four cell lines was investigated and the housekeeping gene expression between cfNAs and mRNA of the cell lines was observed in order to elucidate their relationship
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Aucamp, J. et al. 2016. A quantitative assessment of cell-free DNA utilizing several housekeeping genes: measurements from four different cell lines. (In Gahan. P.B., Fleischhacker M. & Schmidt B., eds. Circulating nucleic acids in serum and plasma – CNAPS IX. Advances in experimental medicine and biology, 924: 101-103. [https://doi.org/10.1007/978-3-319-42044-8_19]