Die ultrastrukturele histopatologie van Exserohilum pedicellatum (Henry) Leonard & Suggs tydens die infeksie van mieliewortels

Abstract

THE ULTRASTRUCTURAL HISTOPATHOLOGY OF EXSEROHILUM PEDICELLATUM (HENRY) LEONARD & SUGGS DURING INFECTION OF MAIZE ROOTS. The aim of this study has been to determine the interaction between the fungus pathogen and the host plant on an ultrastructural level. This pathogen is part of the root rot complex of maize. Heat treated and surface sterilized seeds were planted in pathogen infested vermiculite in pots and also in custom made root inoculation devices. In addition to TEM, SEM and light microscopy , fluorescence microscopy as well as a histochemical procedure for the location of cellulase in infected root tissue, were employed to study the effect of root infection by the pathogen. The maize roots were , apart from microscopical techniques, exposed to an extracted fungus metabolite and commercial cellulase and pectinase in order to obtain more knowledge about the pathogen-host interaction. ...