Description and molecular characterisation of a new nematode species parasitic in the lungs of Strongylopus grayii (Smith) (Anura: Pyxicephalidae) in South Africa

Abstract

Rhabdias delangei n. sp. (Nematoda: Rhabdiasidae) is described from the lungs of the clicking stream frog Strongylopus grayii (Smith) in the Western Cape Province of South Africa. The new species is characterised by the nerve-ring being located just posterior to the mid-length of the oesophagus, a pre-equatorial vulva, as well as a thin-walled and apparently underdeveloped rectum. Pairwise analyses based on ITS-28S and partial cox1 gene fragments showed significant differences between R. delangei n. sp. and its congeners from the Afrotropical Realm. Rhabdias delangei n. sp. is the 12th species of Rhabdias Stiles & Hassall, 1905 recorded in Afrotropical amphibians and the second species of the genus found in pyxicephalid hosts. Our phylogenetic analysis based on the ITS-28S region showed that the new species is closely related to the European species R. rubrovenosa (Schneider, 1866). Furthermore, both species are similar with respect to the underdeveloped rectum and pre-equatorial position of vulva