Elektrofisiologiese eienskappe van Parabuthus transvaalicus venoom op natrium- en kaliumkanale in hart en senuwee

Abstract

The primary aim of this study was to investigate the electrophysiological mechanisms of toxins (PBTx1, Toxin 1, and Toxin 2) isolated from the venom of the scorpion Parabuthus transvaalicus . The research compared the effects of Toxin 1 and 2 on cloned human heart sodium channels (hH1) with native channels in rat heart to determine if animal models could accurately reflect human responses . Key findings showed that native rat heart channels are effective for studying toxin effects . The study identified a specific α-effect induced by Toxin 1 and 2, which arises from two new toxin structures that are smaller than classic sodium channel toxins . A kinetic model was developed showing channels moving in parallel pathways, where the toxins exert negative cooperative effects on transitions to the open state and concentration-dependent effects on inactivation . Both the potassium and sodium toxins were classified into new subfamilies due to their unique structures