Effect of various carbon source, temperature and pH on nitrate reduction efficiency in mineral salt medium enriched with Bacillus weinstephnisis (DS45)

Abstract

The potential bacterial isolates which reduces nitrate using mineral salt medium (MSM) was studied through a batch mode process. Specific nitrate reducing bacteria were isolated from Dharmapuri district (Tamilnadu, India) soil samples. The selected potential bacterium was morphologically and biochemically identified as a Bacillus weinstephnisis (DS45), since it demonstrates more efficiency in removal of nitrate. The effect of various carbon sources, temperatures and pH on removal of 100 mg/L of nitrate was checked by amending the MSM. A rapid nitrate reduction (83.05%) was observed in 0.5% of starch, 35 °C of temperature in pH 7. This study evaluated that the 0.5% starch could be a potential carbon source to enhance the bacterial growth for the removal of nitrate in the aqueous medium within the suitably optimized conditions.