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Development and validation of the simultaneous determination of artemisone, clofazimine and decoquinate with HPLC

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dc.contributor.authorDu Preez, J.L.
dc.contributor.authorAucamp, M.E.
dc.contributor.authorBurger, C.
dc.contributor.authorGerber, M.
dc.contributor.authorViljoen, J.M.
dc.contributor.authorVan Zyl, L.
dc.contributor.authorDu Plessis, J.
dc.contributor.researchID11927860 - Aucamp, Marique Elizabeth
dc.contributor.researchID10065318 - Du Plessis, Jeanetta
dc.contributor.researchID10060510 - Du Preez, Jan Lourens
dc.contributor.researchID11329025 - Gerber, Minja
dc.contributor.researchID21661308 - Burger, Cornel
dc.contributor.researchID11320036 - Viljoen, Johanna Magdalena
dc.contributor.researchID20855125 - Van Zyl, Lindi
dc.date.accessioned2018-04-11T12:10:15Z
dc.date.available2018-04-11T12:10:15Z
dc.date.issued2018
dc.description.abstractThe aim of this study was to develop and validate a novel HPLC method for the simultaneous analysis of artemisone, clofazimine and decoquinate. Detection was obtained at two wavelengths; 284 nm (clofazimine) and 210 nm (artemisone and decoquinate). Gradient elution was used with mobile phase A (A) consisting of 0.005 M sodium octanesulphonic-acid (pH 3.5) and mobile phase B (B) of HPLC grade acetonitrile. The flow rate was set to 1.0 ml/min with (A) at 35% and (B) at 65% for 2 min, followed by a gradient shift of 10/90% ((A)/(B)) over a duration of 4 min. After 10 min, the initial gradient conditions were readjusted to 35/65% ((A)/(B)). Distinctive peaks were identified for clofazimine, artemisone and decoquinate, respectively. The proposed HPLC assay method was validated and found to be reliable, reproducible and accurate for simultaneous analysis of the three compoundsen_US
dc.identifier.citationDu Preez, J.L. et al. 2018. Development and validation of the simultaneous determination of artemisone, clofazimine and decoquinate with HPLC. Die Pharmazie, 73(3):139-142. [https://doi.org/10.1691/ph.2018.7127]en_US
dc.identifier.issn0031-7144
dc.identifier.urihttp://hdl.handle.net/10394/26679
dc.identifier.urihttp://www.ingentaconnect.com/contentone/govi/pharmaz/2018/00000073/00000003/art00004
dc.identifier.urihttps://doi.org/10.1691/ph.2018.7127
dc.language.isoenen_US
dc.publisherAvoxaen_US
dc.titleDevelopment and validation of the simultaneous determination of artemisone, clofazimine and decoquinate with HPLCen_US
dc.typeArticleen_US

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