Morphological modification of Chromolaena odorata cellulosic biomass using alkaline peroxide oxidation pretreatment methodology and its enzymatic conversion to biobased products

Abstract

In this study, the structural modification of Siam weed (Chromolaena odorata) was performed using NaOH-H2O2- and Ca(OH)2-H2O2-based oxidative pretreatment for the enzymatic conversion of the biomass to a biocommodity, reducing sugar (RS). Pretreatment of raw sample was evaluated at temperatures of 60°C, 70°C, 80°C, 90°C for different time intervals of 3, 6, 9, 12 h in alkaline medium (NaOH or Ca (OH)2). The effects of pretreatment time and temperaturewere considered in obtaining the optimum conditions. The optimumconditions for NaOH-H2O2 was obtained at 70°C and 3 h with a maximum cellulose content of 44.29%(w/w), lignin content reduced to 21.09% from the initial raw value of 24.2%. Pretreatment with Ca(OH)2-H2O2 resulted in the optimum conditions obtained to be 70°C for 3 h with a cellulose content of 47.18%. Enzymatic hydrolysis on the pretreated biomass at the optimum conditions showed NaOH-H2O2-treated sample yielded 424.35 mg equivalent glucose/g biomass of RS while Ca(OH)2-H2O2-treated sample yielded 335.81 mg equivalent glucose/g biomass of RS. The untreated raw sample yielded 68.75 mg equivalent glucose/g biomass of RS. Consequently, NaOH-H2O2 pretreatment displayed a higher efficiency than Ca(OH)2-H2O2 pretreatment. Stereomicroscopic and scanning electron microscopic imaging of the treated and untreated samples revealed morphological disruptions brought about by the treatments