DNA barcoding of the supergiant isopods from Bathynomus kensleyi Lowry & Dempsey, 2006 (Cirolanidae) and a molecular biology comparison of B. jamesi Kou, Chen & Li, 2017

Abstract

DNA was extracted from tissue samples from specimens of newly-collected Bathynomus kensleyi from Queensland and subsequently the COI and 16S rRNA sequences were successfully cloned. The holotype of B. kensleyi was also sampled for COI only. Comparison of the sequences showed that, for the COI sequences, B. jamesi and B. kensleyi have more than 59 different DNA positions amongst 596 known reading sequences. The Kimura two parameter (K2P) distance analysis confirmed that B. jamesi and B. kensleyi are two species. Indian records of Bathynomus are reviewed and three of the four identified species from India are shown to be misidentifications. Bathynomus decemspinosus, B. doederlini and B. kensleyi are found to not occur in India and the only accepted record is that of Bathynomus keablei Lowry & Dempsey, 2006. We conclude that based on molecular analysis and morphological comparisons, the correct species identity of Indian species other than Bathynomus keablei remains unknown.