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Genetic characterisation of novel G29P[14] and G10P[11] rotavirus strains from African buffalo

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dc.contributor.authorStrydom, Amy
dc.contributor.authorPotgieter, A. Christiaan
dc.contributor.authorDonato, Celeste
dc.contributor.authorPeenze, Ina
dc.contributor.authorSeheri, Mapaseka
dc.contributor.researchIDArtiodactyl backbone
dc.date.accessioned2020-07-31T09:22:59Z
dc.date.available2020-07-31T09:22:59Z
dc.date.issued2020
dc.description.abstractWe report the first description of rotavirus A strains in African buffalo (Syncerus caffer). Following RNA extraction from stool samples, cDNA was prepared, followed either by sequence-independent amplification and 454 pyrosequencing or direct sequencing on an Illumina MiSeq platform. RVA/Buffalo-wt/ZAF/4426/2002/G29P[14] exhibited a novel G29P[14] combination and an artiodactyl backbone: I2-R2-C2-M2-A11-N2-T6-E2-H3. RVA/Buffalo-wt/ZAF/1442/2007/G10P[11] also exhibited an artiodactyl backbone: I2-R2-C2-M2-A13-N2-T6-E2-H3. Characterisation of these genome constellations indicate that the two buffalo strains are moderately diverse from each other and related to South African bovine RVA strains. The detection of RVA in buffalo contribute to our understanding of the host range of rotavirus in animalsen_US
dc.identifier.citationStrydom, A. et al. 2020. Genetic characterisation of novel G29P[14] and G10P[11] rotavirus strains from African buffalo. Infection, genetics and evolution, 85: #104463. [https://doi.org/10.1016/j.meegid.2020.104463]en_US
dc.identifier.issn1567-1348
dc.identifier.urihttp://hdl.handle.net/10394/35431
dc.identifier.urihttps://www.sciencedirect.com/science/article/abs/pii/S156713482030294X
dc.identifier.urihttps://doi.org/10.1016/j.meegid.2020.104463
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.subjectRotavirus type Aen_US
dc.subjectAfrican buffaloen_US
dc.subjectG29P[14]en_US
dc.subjectArtiodactyl backboneen_US
dc.titleGenetic characterisation of novel G29P[14] and G10P[11] rotavirus strains from African buffaloen_US
dc.typeArticleen_US

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