|dc.contributor.author||Van Scheltinga, Mariska||
|dc.description||Thesis (M.Sc. (Pharmaceutical Chemistry))--North-West University, Potchefstroom Campus, 2006.||
|dc.description.abstract||The objective of the study was to extract lutein, an active component of marigold
flower (Tagetes erecta) by means of supercritical carbon dioxide (sc-C02).
Extractions on freeze-dried marigold petals were performed with a laboratory
scale supercritical fluid extractor (LECO TFEIM2000) of highly advanced design
and performance. Extracts were analysed for lutein by high-performance liquid
chromatography (Agilent Technology 1100 Series HPLC System) using a
calibration line constructed by virtue of solutions of a commercially available
standard (Indofine Chemical Company, Inc.).
The conditions at which a maximum yield of lutein is obtained (52°C, 400 bar, 60
min) were determined by performing runs according to a statistical design and
processing the data by computer assisted surface response analysis. A mass
balance proved effective recovery of the extracted material from the supercritical
The density of sc-C02 was found to be the variable which controls the extraction
as the fluid becomes capable to chemically dissolve lutein once its density (and
thus solvent strength) takes on liquid-like values (between 0.8 and 1.0 g/mL).
The large negative value of the volume of activation ∆*V relates to the decrease
in volume associated with melting and solvation of lutein while it dissolves in the
highly compressed supercritical fluid.
A function combining several process variables into a dimensionless quantity
was used successfully to mathematically describe the extraction process.||
|dc.title||Extraction of lutein from marigold flower with supercritical carbon dioxide||en