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dc.contributor.advisorKotzé, H.F.
dc.contributor.authorGibhard, Liezl
dc.date.accessioned2009-02-17T13:39:20Z
dc.date.available2009-02-17T13:39:20Z
dc.date.issued2007
dc.identifier.urihttp://hdl.handle.net/10394/759
dc.descriptionThesis (M.Sc. (Biochemistry))--North-West University, Potchefstroom Campus, 2008.
dc.description.abstractOzone therapy has been used as a form of alternative medicine but has encountered scepticism by orthodox medicine concerning its effectiveness and toxicity. We assessed the acute and chronic effect of O3 autohaemotherapy (AHT) on liver and muscle damage in baboons. During the acute effect three groups of baboons were used. The first group (n=11) were treated with an O2/O3 gas mixture containing different ozone concentrations i.e. 20, 40 and 80 μg/ml O3 . The second group (n=5) were treated with pure O2 and the third group (n=3) received no treatment and were used to assess the effect of ketamine anaesthesia. O2 and O3-AHT was performed on the baboons by using 5% of their total blood volume. Blood samples were collected in heparin before each treatment and again at 4, 24 and 48 hours. Ketamine anaesthesia caused both liver and muscle damage. Aspartate aminotransferase (AST), alanine aminotransferase (ALT) and creatine kinase (CK) levels increased markedly. O2-AHT had no marked effect on liver and muscle damage and O3-AHT had a protective effect since the increase in AST, ALT and CK levels was not as dramatic as when ketamine alone was used. During the chronic effect O3-AHT were done on 6 baboons, using a O2/O3 gas mixture containing 40 μg/ml O3. Blood were collected before treatment and again at 4, 24, 28, 48, 52, 72 and 96 hours after the first treatment. ALT levels increased during the treatment period of 52 hours and remained elevated for 48 hours following treatment. AST levels increased during the four hours following each treatment and remained elevated for 48 hours following the last treatment. CK levels increased markedly dl-ring the four hours following each treatment, but after treatment was stopped the CK levels decreased dramatically. The magnitude of changes was small and does not support the view that in vitro ozonation of blood is toxic when the treated blood is reinjected back into the baboon. In conclusion, the results do not prove or disprove that 03-AHT caused severe cell damage in baboons. We used 40 μg/ml O3 in the studies where the baboons were treated sequentially (chapter 3). This was because the results obtained with dose of 80 μg/ml O3 were not largely different from that with 40 μg/ml O3 in the acute studies (chapter 2). It was proven in these two studies and also other studies. It was proven in these two studies and also in other studies. I also have no ready explanation of the mechanism through which the liver and muscle were protected by ozone. This has to be investigated.
dc.publisherNorth-West University
dc.titleOzone autohaemotherapy protects against Ketamine hydrochloride® induced liver and muscle damage in baboonsen
dc.typeThesisen
dc.description.thesistypeMasters
dc.contributor.researchID11359250 - Kotzé, Herculaas Frederik (Supervisor)


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