Urinary peptidomics and pulse wave velocity: The African-predict study

Abstract

Increased arterial stiffness is related to early vascular aging and is an independent predictor for cardio vascular disease and mortality. Molecular mechanisms underlying increased arterial stiffness are largely unexplored, especially at the proteome level. We aimed to explore the relationship between pulse wave velocity and urinary proteomics. We included 919 apparently healthy (no chronic illnesses) Black and White men and women (equally distributed) between20 and30 years from the African-PREDICT study. Capillary electrophoresis time-of-flight mass spectrometry was used to analyze the urinary proteome .We measured the carotid-femoral pulse wave velocity to estimate arterial stiffness. In the total group, pulse wave velocity correlated positively with collagen-derived peptides including collagen types I, II, III, IV, V, and IX and inversely with collagen type XI (adjusted for mean arterial pressure). Regarding non collagen-derived peptides, pulse wave velocity positively correlated with polymeric immunoglobulin receptor peptides(n= 2) (allq-value≤0.05).In multi variable adjusted analyses, pulse wave velocity associated positively and independently with seven urinary peptides(collagen typeI, n= 5) (allp-value≤0.05).We found significant positive and independent associations between pulse wave velocity and the collagen typeI-derived peptides, suggesting that dys regulation of collagen typeI in the extra cellular matrix scaffold could lead to early onset of increased arterial stiffness