Exploration of biomarkers for subclinical atherosclerosis in an African population using a proteomics chip targeted at inflammation and cardiovascular disease
Date
2019Author
Jujic, A.
Malan, L.
Mels, C.
Nilsson, P.M.
Magnusson, M.
Metadata
Show full item recordAbstract
Introduction: The evolving use of multiplex proteomic platforms provides
an excellent tool for investigating associations between multiple proteins
and subclinical atherosclerotic disease. In this study, we evaluated the impact of a multiplex protein panel, on carotid intima-media thickness (cIMT)
as a marker of subclinical atherosclerosis.
Purpose: We used a multiplex proteomic platform to identify possible associations between proteins and subclinical carotid atherosclerosis as measured by carotid ultrasound in an African population.
Methods: In the Sympathetic Activity and Ambulatory Blood Pressure
in Africans (SABPA) study, 92 proteins from the Proseek Multiplex CVD
III 96×96 (Olink Bioscience, Sweden) were analyzed in 378 participants
(mean age 44.7±9.6 years, 50.6% women, 10.8% with known cardiovascular disease). Carotid ultrasound was performed for measurements of the
carotid intima-media thickness (cIMT, mean 0.663±0.127 mm) and calculation of cross-sectional wall area (CSWA, mean 13.5±4.4mm2), a measure of target organ damage. Possible associations between the proteins,
and cIMT and CSWA, respectively, were explored using linear regression
models. A two-sided Bonferroni corrected P-value of 0.05/92=5.4x10–4 was
considered statistically significant in the crude analysis.
Results: Of 18 proteins (1 standard deviation of change of ln-transformed
values) that were Bonferroni-corrected (p≤5.4x10–4) significantly associated with cIMT and/or CWAS in crude analyses, the following remained
significant after further adjustment for age, sex, waist circumference, systolic blood pressure, smoking and total cholesterol: growth-differentiation
factor-15 (GDF15; β 0.017, p=0.050), E-selectin (SELE; β 0.019, p=0.017),
carboxypeptidase A1 (CPA1; β 0.019, p=0.019), C-C motif chemokine 15
(CCL15; β 0.031, p<0.001), chitinase-3-like protein 1 (CHI3L1; β 0.021,
p=0.007), the hemoglobin scavenger receptor (CD163; β 0.021, p=0.008)
and osteoprotegerin (OPG; β 0.022, p=0.004). As for target-organ damage
defined by CSWA, SELE (β 0.459, p=0.018), CCL15 (β 0.398; p=0.032)
and CD163 (β 0.541, p=0.005) showed multivariate adjusted significant associations.
Conclusion: In an African population, we could confirm five proteins
(GDF15, SELE, CHI3L1, CD163 and OPG) associated with cIMT, but in
addition identified two proteins (CPA1 and CCL15) with novel associations
with cIMT and/or CSWA
URI
http://hdl.handle.net/10394/34406https://academic.oup.com/eurheartj/article/40/Supplement_1/ehz745.0011/5594827?searchresult=1
https://doi.org/10.1093/eurheartj/ehz745.0011