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    Plasmonic detection of glucose in serum based on biocatalytic shape-altering of gold nanostars

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    Plasmonic_detection.pdf (3.634Mb)
    Date
    2019
    Author
    Phiri, Masauso Moses
    Mulder, Danielle Wingrove
    Vorster, Barend Christiaan
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    Abstract
    Nanoparticles have been used as signal transducers for optical readouts in biosensors. Optical approaches are cost-e ective with easy readout formats for clinical diagnosis. We present a glucose biosensor based on the biocatalytic shape-altering of gold nanostars via silver deposition. Improved sensitivity was observed due to the nanostars clustering after being functionalised with glucose oxidase (GOx). The biosensor quantified glucose in the serum samples with a 1:1000 dilution factor, and colorimetrically distinguished between the concentrations. The assay demonstrated good specificity and sensitivity. The fabricated glucose biosensor is a rapid kinetic assay using a basic entry level laboratory spectrophotometric microplate reader. Such a biosensor could be very useful in resource-constrained regions without state-of-the-art laboratory equipment. Furthermore, naked eye detection of glucose makes this a suitable biosensor for technology transfer to other point-of-care devices
    URI
    http://hdl.handle.net/10394/33214
    https://www.mdpi.com/2079-6374/9/3/83/pdf
    https://doi.org/10.3390/bios9030083
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    • Faculty of Natural and Agricultural Sciences [4311]

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