Attenuation of endoplasmic reticulum stress, impaired calcium homeostasis, and altered bioenergetic functions in MPP+-Exposed SH-SY5Y cells pretreated with rutin
Date
2019Author
Enogieru, Adaze Bijou
Miller, Hayley Christy
Van der Westhuizen, Francois Hendrikus
Haylett, William Lloyd
Hiss, Donavon Charles
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Parkinson’s disease (PD) is a common neurodegenerative disorder that affects approximately 1% of the population over the age of 65 years. While treatment options for PD are limited, reports show that plant-derived bioactive compounds such as rutin possess numerous pharmacological benefits, including antioxidant and antiapoptotic activities. This study aimed to investigate the potential role of rutin in MPP+-treated SH-SY5Y neuroblastoma cells, an established cell model of PD. Our findings reveal increased concentrations of Ca2+ and endoplasmic reticulum (ER) stress as well as impaired mitochondrial membrane potential and bioenergetic status in SH-SY5Y cells treated with MPP+ only. This is demonstrated by a significant reduction in the expression levels of BiP, significantly reduced basal respiration, maximal respiration, and spare respiratory capacity as well as a significant increase in the expression levels of CHOP; however, these effects were significantly attenuated following pretreatment with rutin. Also, rutin significantly improved basal and compensatory glycolysis as a response to an impaired oxidative phosphorylation system triggered by MPP+, characterized by deficient ATP production. In conclusion, our findings provide the first evidence on the ability of rutin to maintain Ca2+ homeostasis, inhibit ER stress, and protect the mitochondria in MPP+-treated SH-SY5Y cells
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http://hdl.handle.net/10394/32445https://link.springer.com/article/10.1007/s12640-019-00048-4
https://doi.org/10.1007/s12640-019-00048-4