Assessment of reproducibility of a VP7 Blocking ELISA diagnostic test for African horse sickness
Date
2019Author
Durán-Ferrer, Manuel
Potgieter, Christiaan
Agüero, Montserrat
Zientara, Stephan
Beck, Cécile
Metadata
Show full item recordAbstract
The laboratory diagnosis of African horse sickness (AHS) is important for: (a) demonstrating freedom from infection in a population, animals or products for trade (b) assessing the efficiency of eradication policies; (c) laboratory confirmation of clinical diagnosis;
(d) estimating the prevalence of AHS infection; and (e) assessing postvaccination
immune status of individual animals or populations. Although serological techniques
play a secondary role in the confirmation of clinical cases, their use is very important for
all the other purposes due to their high throughput, ease of use and good cost‐benefit
ratio. The main objective of this study was to support the validation of AHS VP7 Blocking ELISA up to the Stage 3 of the World Animal Health Organization (OIE) assay validation pathway. To achieve this, a collaborative ring trial, which included all OIE Reference
Laboratories and other AHS‐specialist diagnostic centres, was conducted in order to
assess the diagnostic performance characteristics of the VP7 Blocking ELISA. In this
trial, a panel of sera of different epidemiological origin and infection status was used.
Through this comprehensive evaluation we can conclude that the VP7 Blocking ELISA
satisfies the OIE requirements of reproducibility. The VP7 Blocking ELISA, in its commercial version is ready to enter Stage 4 of the validation pathway (Programme Implementation). Specifically, this will require testing the diagnostic performance of the assay
using contemporary serum samples collected during control campaigns in endemic
countries
URI
http://hdl.handle.net/10394/32080https://onlinelibrary.wiley.com/doi/epdf/10.1111/tbed.12968
https://doi.org/10.1111/tbed.12968