From untargeted LC-QTOF analysis to characterisation of opines in abalone adductor muscle: theory meets practice
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Authors
Venter, Leonie
Jansen van Rensburg, Peet J.
Loots, Du Toit
Lindeque, Jeremie Zander
Vosloo, Andre
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Elsevier
Abstract
Abalone have a unique ability to use pyruvate, various amino acids and dehydrogenases, to produce opines as means to prevent the accumulation of NADH during anaerobic conditions. In this study, the theoretical masses, formulae and fragment patterns of butylated opines were used to predict which of these compounds could be found in the abalone adductor muscle using untargeted liquid chromatography quadrupole time-of flight-mass spectrometry. These findings were validated using synthesised opine standards. In essence alanopine, lysopine, strombine and tauropine produced in abalone adductor muscle could be characterised using the highest identification confidence levels
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Citation
Venter, L. et al. 2017. From untargeted LC-QTOF analysis to characterisation of opines in abalone adductor muscle: theory meets practice. Journal of chromatography B, 1071:44-48. [https://doi.org/10.1016/j.jchromb.2017.01.025]