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dc.contributor.authorOlobatoke, Roseline Y.
dc.contributor.authorMulugeta, Sendros D.
dc.date.accessioned2017-09-27T08:22:11Z
dc.date.available2017-09-27T08:22:11Z
dc.date.issued2015
dc.identifier.citationOlobatoke, R.Y. & Mulugeta, S.D. 2015. Incidence of non-typhoidal Salmonella in poultry products in the North West Province, South Africa. South African Journal of Science, 111(11/12):70–76. [http://dx.doi.org/10.17159/sajs.2015/20140233]en_US
dc.identifier.issn0038-2353
dc.identifier.issn1996-7489 (Online)
dc.identifier.urihttp://hdl.handle.net/10394/25661
dc.identifier.urihttp://dx.doi.org/10.17159/sajs.2015/20140233
dc.description.abstractThis study was conducted to evaluate the incidence of non-typhoidal Salmonella (NTS) serotypes in raw and ready-to-eat (RTE) broiler products in the North West Province of South Africa. A total of 120 raw broiler samples, 40 samples of polonies and 20 samples of smoked viennas were obtained from retail points in major cities and towns in the province. Samples were subjected to aerobic plate count and later screened for the presence of NTS using phenotypic and genotypic techniques. The average bacterial count in raw products was 3.1 x 105 cfu/g whereas bacterial contamination of RTE products was 1.8 x 103 cfu/g. The average recovery rate of NTS species from raw broiler products was 12.5% and the serotypes identified were S. typhimurium (46.4%), S. enteritidis (30.9%) and S. newport (22.9%). No NTS was recovered from the RTE products. However, S. typhimurium was the predominant serotype in whole carcasses whereas S. enteritidis and S. newport were prevalent in chicken parts. Out of the 160 presumptive NTS isolates screened by polymerase chain reaction (PCR), 140 (87.5%) were confirmed for the presence of the Salmonella-specific invA gene. In addition, 115 (82.4%) of the confirmed isolates harboured the plasmid spvC gene. Random Amplified Polymorphic DNA (RAPD) fingerprinting of isolates using RAPD 1 and RAPD 3 primers, revealed some inter- and intra-serotype genetic diversity among isolates, suggesting varying sources of contamination. The results of this study represent the first report on the incidence and prevalent serotypes of NTS in chicken products in the North West Province of South Africa.en_US
dc.language.isoenen_US
dc.publisherAcademy of Science of South Africa (ASSAf)en_US
dc.subjectFood-borne salmonellosisen_US
dc.subjectbroileren_US
dc.subjectinvAen_US
dc.subjectspvCen_US
dc.subjectfingerprintingen_US
dc.titleIncidence of non-typhoidal Salmonella in poultry products in the North West Province, South Africaen_US
dc.typeArticleen_US
dc.contributor.researchID22082085 - Olobatoke, Roseline Yemisi
dc.contributor.researchID20561768 - Mulugeta, Sendros Demeke


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