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    An enquiry concerning the characteristics of cell-free DNA released by cultured cancer cells

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    Date
    2016
    Author
    Bronkhorst, Abel Jacobus
    Wentzel, Johannes F.
    Aucamp, Janine
    Van Dyk, Etresia
    Du Plessis, Lissinda H.
    Pretorius, Piet J.
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    Abstract
    Non-invasive screening that utilizes cell-free DNA (cfDNA) offers remarkable potential as a method for the early detection of genetic disorders and a wide variety of cancers. Unfortunately, one of the most prominent elements delaying the translation of cfDNA analyses to clinical practice is the lack of knowledge regarding its origin and composition. The elucidation of the origin of cfDNA is complicated by the apparently arbitrary variability of quantitative and qualitative characteristics of cfDNA in the blood of healthy as well as diseased individuals. These factors may contribute to false positive/negative results when applied to clinical pathology. Although many have acknowledged that this is a major problem, few have addressed it. We believe that many of the current difficulties encountered in in vivo cfDNA studies can be partially circumvented by in vitro models. The results obtained in this study indicate that the release of cfDNA from 143B cells is not a consequence of apoptosis, necrosis or a product of DNA replication, but primarily the result of actively released DNA, perhaps in association with a protein complex. Moreover, this study demonstrates the potential of in vitro cell culture models to obtain useful information about the phenomenon of cfDNA
    URI
    http://hdl.handle.net/10394/21928
    https://link.springer.com/chapter/10.1007%2F978-3-319-42044-8_4
    https://doi.org/10.1007/978-3-319-42044-8_4
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    • Faculty of Health Sciences [2404]
    • Faculty of Natural and Agricultural Sciences [4855]

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