NWU Institutional Repository

Establishment of different plasmid only-based reverse genetics systems for the recovery of African horse sickness virus

Loading...
Thumbnail Image

Date

Authors

Conradie, Andelé M.
Potgieter, Christiaan A.
Stassen, Liesel
Huisman, Henk
Theron, Jacques

Journal Title

Journal ISSN

Volume Title

Publisher

Elsevier

Abstract

In an effort to simplify and expand the utility of African horse sickness virus (AHSV) reverse genetics, different plasmid-based reverse genetics systems were developed. Plasmids containing cDNAs corresponding to each of the full-length double-stranded RNA genome segments of AHSV-4 under control of a T7 RNA polymerase promoter were co-transfected in cells expressing T7 RNA polymerase, and infectious AHSV-4 was recovered. This reverse genetics system was improved by reducing the required plasmids from 10 to five and resulted in enhanced virus recovery. Subsequently, a T7 RNA polymerase expression cassette was incorporated into one of the AHSV-4 rescue plasmids. This modified 5-plasmid set enabled virus recovery in BSR or L929 cells, thus offering the possibility to generate AHSV-4 in any cell line. Moreover, mutant and cross-serotype reassortant viruses were recovered. These plasmid DNA-based reverse genetics systems thus offer new possibilities for investigating AHSV biology and development of designer AHSV vaccine strains

Description

Citation

Conradie, A.M. et al. 2016. Establishment of different plasmid only-based reverse genetics systems for the recovery of African horse sickness virus. Virology, 499:144-155. [https://www.journals.elsevier.com/virology/]

Endorsement

Review

Supplemented By

Referenced By