• Login
    View Item 
    •   NWU-IR Home
    • Research Output
    • Faculty of Natural and Agricultural Sciences
    • View Item
    •   NWU-IR Home
    • Research Output
    • Faculty of Natural and Agricultural Sciences
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Structural protein VP2 of African Horse Sickness virus is not essential for virus replication in vitro

    Thumbnail
    Date
    2017
    Author
    Van Gennip, René G.P.
    Potgieter, Christiaan A.
    Van Rijn, Piet A.
    Van de Water, Sandra G.P.
    Metadata
    Show full item record
    Abstract
    The Reoviridae family consists of nonenveloped multilayered viruses with a double-stranded RNA genome consisting of 9 to 12 genome segments. The Orbivirus genus of the Reoviridae family contains African horse sickness virus (AHSV), bluetongue virus, and epizootic hemorrhagic disease virus, which cause notifiable diseases and are spread by biting Culicoides species. Here, we used reverse genetics for AHSV to study the role of outer capsid protein VP2, encoded by genome segment 2 (Seg-2). Expansion of a previously found deletion in Seg-2 indicates that structural protein VP2 of AHSV is not essential for virus replication in vitro. In addition, in-frame replacement of RNA sequences in Seg-2 by that of green fluorescence protein (GFP) resulted in AHSV expressing GFP, which further confirmed that VP2 is not essential for virus replication. In contrast to virus replication without VP2 expression in mammalian cells, virus replication in insect cells was strongly reduced, and virus release from insect cells was completely abolished. Further, the other outer capsid protein, VP5, was not copurified with virions for virus mutants without VP2 expression. AHSV without VP5 expression, however, could not be recovered, indicating that outer capsid protein VP5 is essential for virus replication in vitro. Our results demonstrate for the first time that a structural viral protein is not essential for orbivirus replication in vitro, which opens new possibilities for research on other members of the Reoviridae family
    URI
    http://hdl.handle.net/10394/21712
    https://doi.org/10.1128/JVI.01328-16
    http://jvi.asm.org/content/91/4/e01328-16.full.pdf+html
    Collections
    • Faculty of Natural and Agricultural Sciences [4789]

    Copyright © North-West University
    Contact Us | Send Feedback
    Theme by 
    Atmire NV
     

     

    Browse

    All of NWU-IR Communities & CollectionsBy Issue DateAuthorsTitlesSubjectsAdvisor/SupervisorThesis TypeThis CollectionBy Issue DateAuthorsTitlesSubjectsAdvisor/SupervisorThesis Type

    My Account

    LoginRegister

    Copyright © North-West University
    Contact Us | Send Feedback
    Theme by 
    Atmire NV