dc.contributor.author | Van Aardt, Willie J. | |
dc.contributor.author | Le Roux, Jacobus M. | |
dc.contributor.author | Lindeque, Jeremie Z. | |
dc.contributor.author | Mason, Shayne | |
dc.contributor.author | Louw, Roan | |
dc.date.accessioned | 2017-02-01T09:54:57Z | |
dc.date.available | 2017-02-01T09:54:57Z | |
dc.date.issued | 2016 | |
dc.identifier.citation | Van Aardt, W.J. et al. 2016. The effect of temperature on the respiration and metabolism of the African burrowing scorpion (Opistophthalmus latimanus). Comparative biochemistry and physiology, D,20:50-56. [https://doi.org/10.1016/j.cbd.2016.07.003] | en_US |
dc.identifier.issn | 1744-117X | |
dc.identifier.uri | http://hdl.handle.net/10394/19926 | |
dc.identifier.uri | https://doi.org/10.1016/j.cbd.2016.07.003 | |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S1744117X1630065X | |
dc.description.abstract | It is well known that scorpions are highly adapted to thermal temperatures. However, little is known of the metabolic
and respiration adaptations caused by temperature fluctuations in these animals. Therefore we used the
African burrowing scorpion Opistophthalmus latimanus to measure the effect of temperature on its metabolism
and respiration.
Radioactive D-glucose was injected into the ventral sinus of the circulatory system and metabolites of D-glucose
were determined after six hour incubation at four temperatures (7, 17, 25 and 37 °C). The oxygen consumption
rate (ṀO2) and carbon dioxide production rate (ṀCO2) were measured simultaneously at 17, 25 and 37 °C. The
metabolomics investigation included LC-MS, GC–MS and NMR analytical platforms.
The average radioactivity recovered after the carbon-14 D-glucose injection, glycogen precipitation and column
fractionation at the four temperatures was between 92.4% and 95.0%. Strong acids, CO2 and neutral compounds
all increased with temperature, while glycogen and neutral sugars decreased as the temperature increased. Weak
acids initially increased with temperature, then decreased again as the temperature was increased to 37 °C. Respiration
also gradually increased as the temperature was increased. Metabolomics identified 23 metabolites that
were significantly influenced by temperature. Pathway analysis of these metabolites indicated numerous metabolic
pathways that were affected by temperature, clearly demonstrating that the scorpion uses proteins, lipids
and carbohydrates at higher temperatures to generate energy. However, protein catabolism seems to be the
main source of energy at higher temperatures in these animals, although this needs to be confirmed in a more
targeted metabolomics study | en_US |
dc.language.iso | en | en_US |
dc.publisher | Elsevier | en_US |
dc.subject | Metabolic profiling | en_US |
dc.subject | Scorpion | en_US |
dc.subject | Temperature | en_US |
dc.subject | O2 consumption rate | en_US |
dc.subject | CO2 production rate | en_US |
dc.subject | (14-C) carbon-14 D-glucose injection | en_US |
dc.title | The effect of temperature on the respiration and metabolism of the African burrowing scorpion (Opistophthalmus latimanus) | en_US |
dc.type | Article | en_US |
dc.contributor.researchID | 12662275 - Lindeque, Jeremie Zander | |
dc.contributor.researchID | 21487855 - Mason, Shayne William | |
dc.contributor.researchID | 10986707 - Louw, Roan | |
dc.contributor.researchID | 10166920 - Van Aardt, Willem Johannes | |
dc.contributor.researchID | 10211829 - Le Roux, Jacobus Marthinus | |