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    Humoral and cellular immune responses in mice after airway administration of Bacillus thuringiensis Cry1Ab and MON810 cry1Ab–transgenic maize

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    Date
    2015
    Author
    Andreassen, Monica
    Van den Berg, Johnnie
    Rocca, Elena
    Bøhn, Thomas
    Wikmark, Odd-Gunnar
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    Abstract
    Genetically modified (GM) crops may bring new proteins with immunogenic and allergenic properties into the food and feed chains. The most commonly grown GM maize, MON810, expresses a modified version of the insecticidal Cry1Ab protein originating in the soil bacterium Bacillus thuringiensis (Bt). Immune reactions following inhalation of pollen and debris from such plants have been scarcely studied. We exposed BALB/c mice to purified Cry1Ab proteins and Cry1Ab-containing MON810 plant materials by intranasal installation. No anti-Cry1Ab antibodies were detected following exposure to the plant materials. Exposure to purified Cry1Ab resulted in specific anti-Cry1Ab IgG1 and IgE production, indicating inherent immunogenicity and allergenicity. Mice exposed to leaf extracts from both MON810 and unmodified maize demonstrated influx of lymphocytes and eosinophils in the broncho-alveolar lavage, and increased cytokine release in mediastinal lymph node cells. The results indicate that the airway exposure to Cry1Ab proteins may be a route of practical relevance
    URI
    http://hdl.handle.net/10394/18629
    https://doi.org/10.1080/09540105.2014.988128
    http://www.tandfonline.com/doi/full/10.1080/09540105.2014.988128
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    • Faculty of Natural and Agricultural Sciences [4788]

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