In vivo evaluation of the biodistribution and safety of PLGA nanoparticles as drug delivery systems
Date
2010Author
Semete, Boitumelo
Booysen, Laetitia
Lemmer, Yolandy
Kalombo, Lonji
Katata, Lebogang
Verschoor, Jan
Swai, Hulda S.
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Show full item recordAbstract
The remarkable physicochemical properties of particles in the nanometer range have been proven to address many challenges in the
field of science. However, the possible toxic effects of these particles have raised some concerns. The aim of this article is to evaluate the
effects of poly(lactide-co-glycolide) (PLGA) nanoparticles in vitro and in vivo compared to industrial nanoparticles of a similar size range
such as zinc oxide, ferrous oxide, and fumed silica. An in vitro cytotoxicity study was conducted to assess the cell viability following
exposure to PLGA nanoparticles. Viability was determined by means of a WST assay, wherein cell viability of greater than 75% was
observed for both PLGA and amorphous fumed silica particles and ferrous oxide, but was significantly reduced for zinc oxide particles. In
vivo toxicity assays were performed via histopathological evaluation, and no specific anatomical pathological changes or tissue damage
was observed in the tissues of Balb/C mice. The extent of tissue distribution and retention following oral administration of PLGA
particles was analyzed for 7 days. After 7 days, the particles remained detectable in the brain, heart, kidney, liver, lungs, and spleen. The
results show that a mean percentage (40.04%) of the particles were localized in the liver, 25.97% in the kidney, and 12.86% in the brain.
The lowest percentage was observed in the spleen. Thus, based on these assays, it can be concluded that the toxic effects observed with
various industrial nanoparticles will not be observed with particles made of synthetic polymers such as PLGA when applied in the field of
nanomedicine. Furthermore, the biodistribution of the particles warrants surface modification of the particles to avoid higher particle
localization in the liver.
URI
http://hdl.handle.net/10394/16031http://dx.doi.org/10.1016/j.nano.2010.02.002
http://www.journals.elsevier.com/nanomedicine-nanotechnology-biology-and-medicine/
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- Faculty of Health Sciences [2377]