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dc.contributor.authorMarais, Etienne
dc.contributor.authorHamman, Josias
dc.contributor.authorDu Plessis, Lissinda
dc.contributor.authorLemmer, Righard
dc.contributor.authorSteenekamp, Jan
dc.date.accessioned2015-10-13T07:45:19Z
dc.date.available2015-10-13T07:45:19Z
dc.date.issued2013
dc.identifier.citationMarais, E. et al. 2013. Eudragit® L100/N-Trimethylchitosan chloride microspheres for oral insulin delivery. Molecules, 18:6734-6747. [https://doi.org/10.3390/molecules18066734]en_US
dc.identifier.issn1420-3049
dc.identifier.urihttp://hdl.handle.net/10394/14740
dc.identifier.urihttps://doi.org/10.3390/molecules18066734
dc.identifier.urihttp://www.mdpi.com/1420-3049/18/6/6734
dc.description.abstractEffective oral delivery of protein and peptide drugs remains an active topic in scientific research. In this study, matrix type microspheres were prepared with Eudragit® L100 containing N-trimethylchitosan chloride to improve the permeation of insulin across the intestinal epithelium via the paracellular pathway. Insulin loaded microspheres were initially formulated in accordance with a factorial design (23) and manufactured by means of a single water-in-oil emulsification/evaporation method. Based on external and internal morphology two microsphere formulations were selected from the initial formulations for further investigation in terms of particle size, dissolution behaviour and in vitro insulin transport across excised rat intestinal tissue. The initial eight microsphere formulations exhibited drug loading capacities ranging from 27.9–52.4% with different shapes and internal structures. The two selected microsphere formulations had average particle sizes of 157.3 ± 31.74 μm and 135.7 ± 41.05 μm, respectively, and mean dissolution time values for insulin release of 34.47 and 42.63 min, respectively. In vitro transport of insulin across excised rat intestinal tissue from the two selected microsphere formulations was 10.67–fold and 9.68–fold higher than the control group (insulin alone). The microsphere delivery system prepared from Eudragit® L100 containing N-trimethylchitosan chloride is therefore a promising candidate for effective oral insulin delivery.en_US
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.subjectEudragit® L100en_US
dc.subjectInsulinen_US
dc.subjectMicrospheresen_US
dc.subjectN-trimethylchitosan chlorideen_US
dc.subjectOral peptide deliveryen_US
dc.subjectParacellular transporten_US
dc.titleEudragit® L100/N-trimethylchitosan chloride microspheres for oral insulin deliveryen_US
dc.typeArticleen_US
dc.contributor.researchID20402317 - Marais, Etienne Barend
dc.contributor.researchID10081097 - Hamman, Josias Hendrik
dc.contributor.researchID11948388 - Du Plessis, Lissinda Hester
dc.contributor.researchID10096078 - Steenekamp, Jan Harm
dc.contributor.researchID12932574 - Lemmer, Hendrik Jacobus Righard


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