Browsing by Subject "Glycine conjugation"
Now showing items 1-10 of 10
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AKSM2A en AKSM2B ensiemtoets optimalisering en rekombinante uitdrukking
(North-West University (South Africa). Potchefstroom Campus, 2019)Glisienkonjugasie is ʼn Fase 2-biotransformasieweg wat verantwoordelik is vir die detoksifisering van xenobiotika soos bensoaat en salisilaat. Hierdie biotransformasieweg is gekonserveerd en speel ʼn baie belangrike rol in ... -
Analyses of the genetic diversity and protein expression variation of the acyl: CoA medium-chain ligases, ACSM2A and ACSM2B
(Springer, 2018)Benzoate (found in milk and widely used as preservative), salicylate (present in fruits and the active component of aspirin), dietary polyphenols produced by gut microbiota, metabolites from organic acidemias, and medium-chain ... -
The establishment of a model system in which variants of the medium-chain ligase (ACSM) and glycine N-acyltransferase (GLYAT) can be co-expressed and analysed
(North-West University (South Africa), 2022)Even though glycine conjugation pathway discovery dates back to the 18th century, it is still an important detoxification pathway that is severely neglected in research. Xenobiotics are omnipresent in the environment (as ... -
Glycine conjugation: importance in metabolism, the role of glycine N-acyltransferase, and factors that influence interindividual variation
(Taylor & Francis, 2013)Introduction: Glycine conjugation of mitochondrial acyl-CoAs, catalyzed by glycine N-acyltransferase (GLYAT, E.C. 2.3.1.13), is an important metabolic pathway responsible for maintaining adequate levels of free coenzyme A ... -
Investigation of the bi-substrate kinetics of a recombinant human glycine N-acyltransferase with a known non-synonymous single nucleotide polymorphism (N156S)
(North-West University (South Africa), Potchefstroom Campus, 2015)Biotransformation is fast becoming a buzzword of our time. This is due to the ever increasing exposure of humans to xenobiotic substances. The detoxification of these xenobiotics occur via various detoxifying mechanisms ... -
A new perspective on the importance of glycine conjugation in the metabolism of aromatic acids
(Taylor & Francis, 2014)A number of endogenous and xenobiotic organic acids are conjugated to glycine, in animals ranging from mosquitoes to humans. Glycine conjugation has generally been assumed to be a detoxification mechanism, increasing the ... -
Phase 2 biotransformation : the targeted profiling of glycine amino acid conjugation
(North-West University (South Africa), Potchefstroom Campus, 2015)The hepatic biotransformation system plays an important role in drug metabolism and toxicity and has been studied quite extensively. The importance of the very first biotransformation reaction discovered, namely phase 2 ... -
The recombinant expression and enzymatic charaterisation of human ACSM2 ligase.
(North-West University (South Africa), 2023-10)Glycine conjugation is a highly conserved Phase 2 biotransformation reaction for xenobiotic carboxylic acids such as benzoate, salicylate, and medium-chain fatty acids. The glycine conjugation pathway can be saturated by ... -
The use of p-aminobenzoic acid as a probe substance for the targeted profiling of glycine conjugation
(Wiley, 2016)Glycine conjugation facilitates the metabolism of toxic aromatic acids, capable of disrupting mitochondrial integrity. Owing to the high exposure to toxic substrates, characterization of individual glycine conjugation ... -
Xenobiotic/medium chain fatty acid: CoA ligase - a critical review on its role in fatty acid metabolism and the detoxification of benzoic acid and aspirin
(Taylor & Francis, 2016)Introduction: Activation of fatty acids by the acyl-CoA synthetases (ACSs) is the vital first step in fatty acid metabolism. The enzymatic and physiological characterization of the human xenobiotic/medium chain fatty acid: ...
