Browsing by Subject "Cell-free DNA"
Now showing items 1-10 of 13
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Cell-free DNA in a three-dimensional spheroid cell culture model: a preliminary study
(Elsevier, 2017)Background Investigating the biological functions of cell-free DNA (cfDNA) is limited by the interference of vast numbers of putative sources and causes of DNA release into circulation. Utilization of three-dimensional ... -
Cell-free DNA: preanalytical variables
(Elsevier, 2015)Since the discovery of cell-free DNA (cfDNA) in human blood, most studies have focused on diagnostic and prognostic uses of these markers for solid tumors. Except for some prenatal tests and BEAMing, cfDNA analysis has not ... -
Continuous adaptation through genetic communication: a putative role for cell-free DNA
(Taylor & Francis, 2012)Introduction: The virtosome fraction of cell-free DNA, being newly synthesised, capable of horizontal gene transfer (HGT) and subsequent genomic incorporation and expression, may play crucial roles in the biological functions ... -
Development and validation of a qPCR assay for the non-invasive determination of fetal sex in cattle and African Buffalo
(North-West University (South Africa)North-West University, 2020)The expansive possibilities and research progress of cell-free DNA (cfDNA) and cell-free foetal DNA (cffDNA) have engendered numerous articles published on a regular basis in recent years. However, this has mainly been ... -
Kinetic analysis, size profiling, and bioenergetic association of DNA released by selected cell lines in vitro
(Springer, 2017)Although circulating DNA (cirDNA) analysis shows great promise as a screening tool for a wide range of pathologies, numerous stumbling blocks hinder the rapid translation of research to clinical practice. This is related ... -
Methodological variables in the analysis of cell-free DNA
(Springer, 2016)In recent years, cell-free DNA (cfDNA) analysis has received increasing amounts of attention as a potential non-invasive screening tool for the early detection of genetic aberrations and a wide variety of diseases, especially ... -
Molecular and biological characterization of cell-free DNA using an in vitro cell culture model
(North-West University, Potchefstroom Campus, 2017)Over the past 40 years, numerous proof-of-principle studies have intermittently demonstrated the translational potential of cfDNA as a non-invasive biomarker for the diagnostication, prognostication and therapy monitoring ... -
Origin, translocation and destination of extracellular occurring DNA: a new paradigm in genetic behaviour
(Elsevier, 2011)The diagnostic value of extracellular occurring DNA (eoDNA) is limited by our lack of understanding its biological function. eoDNA exists in a number of forms, namely vesicle bound DNA (apoptotic bodies, micro particles, ... -
A quantitative assessment of cell-free DNA utilizing several housekeeping genes: measurements from four different cell lines
(Springer, 2016)Quantitative real-time PCR (qPCR) is regularly used to quantify cell-free nucleic acids (cfNAs) in order to identify biomarkers for various pathologies. However, studies have shown notable housekeeping gene expression ... -
Reference gene selection for in vitro cell-free DNA analysis and gene expression profiling
(Elsevier, 2016)Objectives: (i) To optimize cell-free DNA (cfDNA) and mRNA quantification using eight housekeeping genes (HKGs), (ii) to determine if there is a difference in the occurrence of HKGs in the cfDNA andmRNA of normal cells and ...
