In vitro antioxidant, antimutagenic and genoprotective activity of Rosa roxburghii fruit extract
Date
2008Author
Van der Westhuizen, Francois H.
Janse van Rensburg, Catharina S.
Rautenbach, George S.
Loots, Du Toit
Huysamen, Cristal
Louw, Roan
Pretorius, Pieter J.
Erasmus, Elardus
Marnewick, Jeanine L.
Metadata
Show full item recordAbstract
The antioxidant properties of the fruit of the Rosa roxburghii (RR) plant have been associated with several
putative health promoting effects. The possible cytotoxic, mutagenic/antimutagenic and genotoxic effects of
RR fruit extract were investigated. The effect on antioxidant status and protection against induced oxidative
stress were also investigated using primary rat hepatocytes. A RR fruit extract containing 45 g/L total ascorbic
acid and 65 g/L total polyphenols was used in this study. Dilutions up to 0.08% (v/v) increased significantly
the antioxidant status in primary rat hepatocytes. The glutathione redox state was decreased with RR treatment
but was increased in Chang liver cells and MT-2 lymphoblast. No cyto- or genotoxicity were observed
at levels of up to 5% (v/v) of the fruit extract. In addition, a significant protection against t-BHP induced
oxidative stress was observed in primary rat hepatocytes. The Ames test revealed no mutagenic activity
using the Salmonella typhimurium strains TA98, TA100 and TA102. A significant antimutagenic effect of
the extract was observed against the metabolic activated mutagens 2-acetylaminofluorene and aflatoxin B1
and to a lesser extent against methyl methanesulfonate. It is concluded that these results support the
associated health promoting potential of Rosa Roxburghii fruit and in particular against oxidative stress
URI
http://hdl.handle.net/10394/2669https://onlinelibrary.wiley.com/doi/abs/10.1002/ptr.2330
https://doi.org/10.1002/ptr.2330