Takeda, KazukiIkenaka, YoshinoriMorita, AyukaNakayama, Shouta M.M.Ishizuka, Mayumi2020-03-192020-03-192020Takeda, K. et al. 2020. Comparison of two reducing agents dithiothreitol and tris(3-hydroxypropyl)phosphine for in vitro kinetic assay of vitamin K epoxide reductase. Veterinary and animal science, 9: #100095. [https://doi.org/10.1016/j.vas.2020.100095]2451-943Xhttp://hdl.handle.net/10394/34418https://www.sciencedirect.com/science/article/pii/S2451943X20300089https://doi.org/10.1016/j.vas.2020.100095Vitamin K epoxide reductase (VKOR) is a target enzyme for anticoagulants, such as warfarin, that are used as medicines or rodenticides. Assessing VKOR activity is required to ensure the proper usage of these drugs. Dithiothreitol (DTT) is a typical disulfide reductant that is used as a substrate for in vitro VKOR assays. However, DTT is considered problematic because of its side effects. Tris(3-hydroxypropyl)phosphine (THP) has been found to be a reliable alternative to DTT, as shown by kinetic analyses of the VKOR with them. THP showed significantly lower Vmax and Km values than those of DTT; however, there was no significant difference in their Vmax/Km and IC50 for warfarinenVitamin KVitamin K epoxide reductaseDithiothreitolTris(3-hydroxypropyl)phosphineAnticoagulantArticle