Thompson, Lesa A.Ikenaka, YoshinoriDarwish, Wageh SobhyNakayama, Shouta M.M.Mizukawa, Hazuki2019-09-202019-09-202019Thompson, L.A. et al. 2019. Effects of the organochlorine p,p’-DDT on MCF-7 cells: investigating metabolic and immune modulatory transcriptomic changes. Environmental toxicology and pharmacology, 72: Article no 103249. [https://doi.org/10.1016/j.etap.2019.103249]1382-66891872-7077 (Online)http://hdl.handle.net/10394/33358https://www.sciencedirect.com/science/article/pii/S1382668919301218https://doi.org/10.1016/j.etap.2019.103249The organochlorine pesticide dichloro-diphenyl-trichloroethane (DDT) is persistent in the environment and leadsto adverse human health effects. High levels in breast milk pose a threat to both breast tissue and nursing infants.The objectives of this study were to investigate DDT-induced transcriptomic alterations in enzymes and trans-porters involved in xenobiotic metabolism, immune responses, oxidative stress markers, and cell growth in ahuman breast cancer cell line. MCF-7 cells were exposed to both environmentally-relevant and previously-testedconcentrations ofp,p’-DDT in a short-term experiment. Significant up-regulation of metabolizing enzymes andtransporters (ACHE,GSTO1,NQO1andABCC2) and oxidative stress markers (CXCL8,HMOX-1,NFE2L2andTNF) was clearly observed. Conversely,UGT1A6,AHRand cell growth genes (FGF2andVEGFA) were severelydown-regulated. Identification of these genes helps to identify mechanisms ofp,p’-DDT action within cells andmay be considered as useful biomarkers for exposure to DDT contaminationenp,p’-DDTMCF-7 cellsBiomarkersTranscriptomicsArticle