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dc.contributor.authorPhiri, Masauso Moses
dc.contributor.authorMulder, Danielle Wingrove
dc.contributor.authorVorster, Barend Christiaan
dc.date.accessioned2019-08-13T13:34:40Z
dc.date.available2019-08-13T13:34:40Z
dc.date.issued2019
dc.identifier.citationPhiri, M.M. et al. 2019. Plasmonic detection of glucose in serum based on biocatalytic shape-altering of gold nanostars. Biosensors, 9(3): Article no 83. [https://doi.org/10.3390/bios9030083]en_US
dc.identifier.issn2079-6374 (Online)
dc.identifier.urihttp://hdl.handle.net/10394/33214
dc.identifier.urihttps://www.mdpi.com/2079-6374/9/3/83/pdf
dc.identifier.urihttps://doi.org/10.3390/bios9030083
dc.description.abstractNanoparticles have been used as signal transducers for optical readouts in biosensors. Optical approaches are cost-e ective with easy readout formats for clinical diagnosis. We present a glucose biosensor based on the biocatalytic shape-altering of gold nanostars via silver deposition. Improved sensitivity was observed due to the nanostars clustering after being functionalised with glucose oxidase (GOx). The biosensor quantified glucose in the serum samples with a 1:1000 dilution factor, and colorimetrically distinguished between the concentrations. The assay demonstrated good specificity and sensitivity. The fabricated glucose biosensor is a rapid kinetic assay using a basic entry level laboratory spectrophotometric microplate reader. Such a biosensor could be very useful in resource-constrained regions without state-of-the-art laboratory equipment. Furthermore, naked eye detection of glucose makes this a suitable biosensor for technology transfer to other point-of-care devicesen_US
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.subjectGlucose biosensoren_US
dc.subjectGold nanostarsen_US
dc.subjectColorimetric detectionen_US
dc.subjectGlucose oxidaseen_US
dc.subjectLocalized surface plasmon resonanceen_US
dc.subjectBiocatalytic shape-alteringen_US
dc.titlePlasmonic detection of glucose in serum based on biocatalytic shape-altering of gold nanostarsen_US
dc.typeArticleen_US
dc.contributor.researchID25264834 - Phiri, Masauso Moses
dc.contributor.researchID26718944 - Mulder, Danielle Wingrove
dc.contributor.researchID22713077 - Vorster, Barend Christiaan


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