dc.contributor.author | De Menezs, Elaine C. Soares | |
dc.contributor.author | Elson, Joanna L. | |
dc.contributor.author | Brown, Audrey E. | |
dc.contributor.author | Bowman, Simon J. | |
dc.contributor.author | Mirza, Kamran | |
dc.date.accessioned | 2019-04-17T07:35:37Z | |
dc.date.available | 2019-04-17T07:35:37Z | |
dc.date.issued | 2018 | |
dc.identifier.citation | De Menezes, E.C.S. et al. 2018. Mitochondrial DNA copy number is not associated with fatigue status in Primary Sjögren’s Syndrome. Fatigue: biomedicine, health and behavior, 6(3):123-131. [https://doi.org/10.1080/21641846.2018.1486799] | en_US |
dc.identifier.issn | 2164-1846 | |
dc.identifier.issn | 2164-1862 (Online) | |
dc.identifier.uri | http://hdl.handle.net/10394/32233 | |
dc.identifier.uri | https://www.tandfonline.com/doi/full/10.1080/21641846.2018.1486799 | |
dc.identifier.uri | https://doi.org/10.1080/21641846.2018.1486799 | |
dc.description.abstract | Background: Primary Sjögren’s syndrome (pSS) is a heterogeneous disease characterized by lymphocytic infiltrates to the exocrine glands, causing sicca symptoms and other manifestations. Fatigue is one of the most prominent symptom in pSS; up to 70% suffer from chronic fatigue. Fatigue has been shown to be common and severe in patients suffering from primary mitochondrial DNA (mtDNA) disease. In a number of chronic diseases, mitochondrial DNA copy number (mtDNAcn) has been reported to be altered.
Purpose: The aim of the study was to examine if mtDNAcn was altered in fatigued versus non-fatigued pSS.
Methods: We quantified mtDNAcn using quantitative polymerase chain reaction (qPCR) with mitochondrial ND2 as a target gene normalized to nuclear HβB (mtDNA:nDNA).
Results: In 204 participants, 108 fatigued and 96 non-fatigued, relative mtDNAcn did not distinguish fatigue status (p = 0.7) nor was it correlated with severity of fatigue (p = 0.21).
Conclusions: MtDNAcn is not altered with fatigue status in blood in pSS. Our analysis suggests that when conducting mtDNAcn analysis with large sample numbers over multiple days a two-way-ANOVA should be used in preference to a one-way-ANOVA or t-test to allow detection of batch effects | en_US |
dc.language.iso | en | en_US |
dc.publisher | Taylor & Francis | en_US |
dc.subject | Mitochondria | en_US |
dc.subject | Mitochondrial DNA copy number (mtDNAcn) | en_US |
dc.subject | Fatigue | en_US |
dc.subject | Primary Sjögren’s Syndrome | en_US |
dc.subject | mtDNA | en_US |
dc.title | Mitochondrial DNA copy number is not associated with fatigue status in Primary Sjögren’s Syndrome | en_US |
dc.type | Article | en_US |
dc.contributor.researchID | 24952338 - Elson, Joanna L. | |