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dc.contributor.authorDe Menezs, Elaine C. Soares
dc.contributor.authorElson, Joanna L.
dc.contributor.authorBrown, Audrey E.
dc.contributor.authorBowman, Simon J.
dc.contributor.authorMirza, Kamran
dc.date.accessioned2019-04-17T07:35:37Z
dc.date.available2019-04-17T07:35:37Z
dc.date.issued2018
dc.identifier.citationDe Menezes, E.C.S. et al. 2018. Mitochondrial DNA copy number is not associated with fatigue status in Primary Sjögren’s Syndrome. Fatigue: biomedicine, health and behavior, 6(3):123-131. [https://doi.org/10.1080/21641846.2018.1486799]en_US
dc.identifier.issn2164-1846
dc.identifier.issn2164-1862 (Online)
dc.identifier.urihttp://hdl.handle.net/10394/32233
dc.identifier.urihttps://www.tandfonline.com/doi/full/10.1080/21641846.2018.1486799
dc.identifier.urihttps://doi.org/10.1080/21641846.2018.1486799
dc.description.abstractBackground: Primary Sjögren’s syndrome (pSS) is a heterogeneous disease characterized by lymphocytic infiltrates to the exocrine glands, causing sicca symptoms and other manifestations. Fatigue is one of the most prominent symptom in pSS; up to 70% suffer from chronic fatigue. Fatigue has been shown to be common and severe in patients suffering from primary mitochondrial DNA (mtDNA) disease. In a number of chronic diseases, mitochondrial DNA copy number (mtDNAcn) has been reported to be altered. Purpose: The aim of the study was to examine if mtDNAcn was altered in fatigued versus non-fatigued pSS. Methods: We quantified mtDNAcn using quantitative polymerase chain reaction (qPCR) with mitochondrial ND2 as a target gene normalized to nuclear HβB (mtDNA:nDNA). Results: In 204 participants, 108 fatigued and 96 non-fatigued, relative mtDNAcn did not distinguish fatigue status (p = 0.7) nor was it correlated with severity of fatigue (p = 0.21). Conclusions: MtDNAcn is not altered with fatigue status in blood in pSS. Our analysis suggests that when conducting mtDNAcn analysis with large sample numbers over multiple days a two-way-ANOVA should be used in preference to a one-way-ANOVA or t-test to allow detection of batch effectsen_US
dc.language.isoenen_US
dc.publisherTaylor & Francisen_US
dc.subjectMitochondriaen_US
dc.subjectMitochondrial DNA copy number (mtDNAcn)en_US
dc.subjectFatigueen_US
dc.subjectPrimary Sjögren’s Syndromeen_US
dc.subjectmtDNAen_US
dc.titleMitochondrial DNA copy number is not associated with fatigue status in Primary Sjögren’s Syndromeen_US
dc.typeArticleen_US
dc.contributor.researchID24952338 - Elson, Joanna L.


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