dc.contributor.author | Conradie, Andelé M. | |
dc.contributor.author | Potgieter, Christiaan A. | |
dc.contributor.author | Stassen, Liesel | |
dc.contributor.author | Huisman, Henk | |
dc.contributor.author | Theron, Jacques | |
dc.date.accessioned | 2017-05-08T13:54:49Z | |
dc.date.available | 2017-05-08T13:54:49Z | |
dc.date.issued | 2016 | |
dc.identifier.citation | Conradie, A.M. et al. 2016. Establishment of different plasmid only-based reverse genetics systems for the recovery of African horse sickness virus. Virology, 499:144-155. [https://www.journals.elsevier.com/virology/] | en_US |
dc.identifier.issn | 0042-6822 | |
dc.identifier.uri | http://hdl.handle.net/10394/21753 | |
dc.identifier.uri | https://doi.org/10.1016/j.virol.2016.07.010 | |
dc.identifier.uri | http://www.sciencedirect.com/science/article/pii/S0042682216301751 | |
dc.description.abstract | In an effort to simplify and expand the utility of African horse sickness virus (AHSV) reverse genetics, different plasmid-based reverse genetics systems were developed. Plasmids containing cDNAs corresponding to each of the full-length double-stranded RNA genome segments of AHSV-4 under control of a T7 RNA polymerase promoter were co-transfected in cells expressing T7 RNA polymerase, and infectious AHSV-4 was recovered. This reverse genetics system was improved by reducing the required plasmids from 10 to five and resulted in enhanced virus recovery. Subsequently, a T7 RNA polymerase expression cassette was incorporated into one of the AHSV-4 rescue plasmids. This modified 5-plasmid set enabled virus recovery in BSR or L929 cells, thus offering the possibility to generate AHSV-4 in any cell line. Moreover, mutant and cross-serotype reassortant viruses were recovered. These plasmid DNA-based reverse genetics systems thus offer new possibilities for investigating AHSV biology and development of designer AHSV vaccine strains | en_US |
dc.language.iso | en | en_US |
dc.publisher | Elsevier | en_US |
dc.subject | African horse sickness virus | en_US |
dc.subject | Orbivirus | en_US |
dc.subject | Reverse genetics | en_US |
dc.subject | dsRNA | en_US |
dc.subject | Reoviridae | en_US |
dc.subject | Virus rescue | en_US |
dc.subject | T7 RNA polymerase | en_US |
dc.subject | Reassortment | en_US |
dc.title | Establishment of different plasmid only-based reverse genetics systems for the recovery of African horse sickness virus | en_US |
dc.type | Article | en_US |
dc.contributor.researchID | 10085637 - Potgieter, Abraham Christiaan | |