Characterization and tissue distribution of conjugated metabolites of pyrene in the rat
Date
2015Author
Saengtienchai, Aksorn
Ikenaka, Yoshinori
Darwish, Wageh Sobhy
Nakayama, Shouta M.M.
Mizukawa, Hazuki
Metadata
Show full item recordAbstract
Pyrene (PY) is a polycyclic aromatic hydrocarbon (PAH) that is often used as a biomarker for human and wildlife exposure to
PAHs. As the metabolites of PAHs, similar to their parent compounds, pose public health risks, it is necessary to study their characteristics
and tissue-specific distribution. The present study was performed to experimentally characterize PY metabolites and analyze the tissuespecific
distribution of the conjugated metabolites after oral administration of PY to rats. PY metabolites, such as pyrenediol-disulfate
(PYdiol-diS), pyrenediol-sulfate (PYdiol-S), pyrene-1-sufate (PYOS), pyrene-1-glucuronide (PYOG) and 1-hydroxypyrene (PYOH), were
detected in rat urine. Although glucuronide conjugate was the predominant metabolite, the metabolite composition varied among tissues.
Interestingly, the proportion of PYOH was high in the large intestine. Furthermore, PYOH was the only PY metabolite detected in feces.
KEY WORDS: conjugated metabolites, glucuronidation, rat, sulfation, tissue distribution
URI
http://hdl.handle.net/10394/18821http://doi.org/10.1292/jvms.14-0632
https://www.jstage.jst.go.jp/article/jvms/77/10/77_14-0632/_article