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    Morphological and molecular studies on Aphelenchoides arachidis Bos, 1977 (Tylenchina: Aphelenchoididae) from groundnuts in South Africa 

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    Date
    2015
    Author
    Lesufi, Moses M.
    McDonald, Alex H.
    Tiedt, Louwrens R.
    Swart, Antoinette
    Knoetze, Rinus
    Truter, Mariette
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    Abstract
    Aphelenchoides arachidis is reported for the first time from South Africa and for the fourth time outside Nigeria. The A. arachidis-infested pods from South Africa showed the following symptoms: small seeds with the testa wrinkled and darker in colour than that of non-infested seeds; the pods showed dark lesions and some seeds within the pods showed early germination. Differences between the two South African and the Nigerian populations of A. arachidis include more lateral lines in some specimens (2-4 vs 2) and, on average, longer post-uterine sac length (extending for 74 (41-96) and 62 (33-82) vs about 50% of vulva to anus distance). Scanning electron micrographs of this species are presented for the first time. The ITS regions of ribosomal DNA were amplified, sequenced, aligned and compared with other sequences of Aphelenchoides species. Two pathogenic fungi, Thielaviopsis basicola and Neocosmospora vasinfecta, were also isolated from this material
    URI
    http://hdl.handle.net/10394/18757
    http://booksandjournals.brillonline.com/content/journals/10.1163/15685411-00002879
    http://dx.doi.org/10.1163/15685411-00002879
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