dc.contributor.author | Jere, Khuzwayo C. | |
dc.contributor.author | Mlera, Luwanika | |
dc.contributor.author | Van Dijk, Alberdina A. | |
dc.contributor.author | O'Neill, Hester G. | |
dc.contributor.author | Peenze, Ina | |
dc.date.accessioned | 2016-05-31T12:34:54Z | |
dc.date.available | 2016-05-31T12:34:54Z | |
dc.date.issued | 2012 | |
dc.identifier.citation | Jere, K.C. et al. 2012. Whole genome sequence analyses of three African bovine rotaviruses reveal that they emerged through multiple reassortment events between rotaviruses from different mammalian species. Veterinary microbiology, 159:245-250. [https://doi.org/10.1016/j.vetmic.2012.03.040] | en_US |
dc.identifier.issn | 0378-1135 | |
dc.identifier.issn | 1873-2542 (Online) | |
dc.identifier.uri | http://hdl.handle.net/10394/17554 | |
dc.identifier.uri | https://doi.org/10.1016/j.vetmic.2012.03.040 | |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S0378113512002076 | |
dc.description.abstract | Animal-to-human interspecies transmission is one of the evolutionary mechanisms
driving rotavirus strain diversity in humans. Although quite a few studies emanating from
Africa revealed evidence of bovine-to-human rotavirus interspecies transmission, whole
genome data of African bovine rotavirus strains are not yet available. To gain insight into
the complete genome constellation of African bovine rotaviruses, the full genomes of three
bovine rotavirus strains were extracted from stool samples collected from calves,
amplified using a sequence-independent procedure, followed by 4541 pyrosequencing.
Strains RVA/Cow-wt/ZAF/1603/2007/G6P[5] and RVA/Cow-wt/ZAF/1605/2007/G6P[5]
were both genotyped as G6-P[5]-I2-R2-C2-M2-A3-N2-T6-E2-H3 and were probably
two variants of the same rotavirus due to their close nucleotide sequence similarity. The
genotype constellation of strain RVA/Cow-wt/ZAF/1604/2007/G8P[1] was G8-P[1]-I2-R2-
C2-M2-A3-N2-T6-E2-H3. The genetic relationships and phylogenetic analyses suggested
that these three bovine rotavirus strains may have emerged through multiple
reassortment events between bovine, giraffe and antelope rotaviruses. Due to the close
relatedness of genome segments 1 (encoding VP1), 7 (NSP2), 9 (VP7) and 10 (NSP4) of
strain RVA/Cow-wt/ZAF/1604/2007/G8P[1] to those of the corresponding segments of
human rotaviruses, RVA strain 1604 may represent bovine strains that were transmitted to
humans and possibly reassorted with human rotaviruses previously. The complete
nucleotide sequences of the bovine rotavirus strains reported in this study represent the
first whole genome data of bovine rotaviruses from Africa | en_US |
dc.description.sponsorship | North-West University, the South African National
Research Foundation (grants FA2005031700015 and UID
63427), the Poliomyelitis Research Foundation of South
Africa (grant no. 09/34) and Cannon Collins Trust | en_US |
dc.language.iso | en | en_US |
dc.publisher | Elsevier | en_US |
dc.subject | Bovine rotavirus | en_US |
dc.subject | 454 pyrosequencing | en_US |
dc.subject | Whole genome analysis | en_US |
dc.subject | Sequence-independent cDNA synthesis and amplification | en_US |
dc.title | Whole genome sequence analyses of three African bovine rotaviruses reveal that they emerged through multiple reassortment events between rotaviruses from different mammalian species | en_US |
dc.type | Article | en_US |
dc.contributor.researchID | 10997938 - Van Dijk, Alberdina Aike | |
dc.contributor.researchID | 22147322 - Jere, Khuzwayo C. | |
dc.contributor.researchID | 22311890 - Mlera, Luwanika | |
dc.contributor.researchID | 21484317 - O'Neill, Hester Gertruida | |