Dominant inheritance of field–evolved resistance to Bt corn in Busseola fusca

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Date
2013Author
Campagne, Pascal
Kruger, Marlene
Van den Berg, Johnnie
Pasquet, Rémy
Le Ru, Bruno
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Transgenic crops expressing Bacillus thuringiensis (Bt) toxins have been adopted worldwide, notably in developing
countries. In spite of their success in controlling target pests while allowing a substantial reduction of insecticide use,
the sustainable control of these pest populations is threatened by the evolution of resistance. The implementation of
the “high dose/refuge” strategy for managing insect resistance in transgenic crops aims at delaying the evolution of
resistance to Bt crops in pest populations by promoting survival of susceptible insects. However, a crucial condition
for the “high dose/refuge” strategy to be efficient is that the inheritance of resistance should be functionally recessive.
Busseola fusca developed high levels of resistance to the Bt toxin Cry 1Ab expressed in Bt corn in South Africa. To
test whether the inheritance of B. fusca resistance to the Bt toxin could be considered recessive we performed
controlled crosses with this pest and evaluated its survival on Bt and non-Bt corn. Results show that resistance of B.
fusca to Bt corn is dominant, which refutes the hypothesis of recessive inheritance. Survival on Bt corn was not lower
than on non-Bt corn for both resistant larvae and the F1 progeny from resistant × susceptible parents. Hence,
resistance management strategies of B. fusca to Bt corn must address non-recessive resistance
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http://hdl.handle.net/10394/16042http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0069675
http://www.plosone.org/