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dc.contributor.authorVan den Berg, Esther
dc.contributor.authorTiedt, Louwrens R.
dc.contributor.authorCoyne, Daniel L.
dc.contributor.authorPloeg, Antoon T.
dc.contributor.authorNavas-Cortés, Juan A.
dc.date.accessioned2015-11-16T07:45:34Z
dc.date.available2015-11-16T07:45:34Z
dc.date.issued2013
dc.identifier.citationVan den Berg, E. et al. 2013. Morphological and molecular characterisation and diagnostics of some species of Scutellonema Andrássy, 1958 (Tylenchida: Hoplolaimidae) with a molecular phylogeny of the genus. Nematology, 15(6):719-745. [http://www.ingentaconnect.com/content/brill/nemy]en_US
dc.identifier.issn1388-5545
dc.identifier.issn1568-5411 (Online)
dc.identifier.urihttp://hdl.handle.net/10394/15066
dc.description.abstractScutellonema spp. are widely distributed across tropical and subtropical regions of the world and are associated with numerous agricultural and horticultural crops. Identification of many Scutellonema species is not always reliable, in part because many species share very similar diagnostic characters. In this study, we provide morphological and molecular characterisation of S. brachyurus from the USA and South Africa, S. bradys from Nigeria and three unidentified species from California, USA, New Zealand and Burkina Faso. Morphological descriptions, measurements, light and scanning electron microscopic photos and drawings are given for S. brachyurus. Females of S. brachyurus from the USA (type A) and South Africa (type B) showed a significant variation in the number of sectors and blocks on the lip annuli, ranging from about 4-12 and from 8-20, respectively. Molecular analysis using the D2- D3 of 28S rRNA, ITS rRNA and the COI mtDNA gene sequences revealed two distinct genotypes within S. brachyurus samples: type A (samples from USA, Italy, Korea, Taiwan) and type B (South Africa). Multivariate analyses determined that S. brachyurus from the USA and Taiwan (type A) differed from that from South Africa (type B) mainly in body, tail and DGO lengths, and ratios b , c , c and V. Phylogenetic relationships within Scutellonema are given as inferred from the analyses of the D2-D3 of 28S rRNA, ITS rRNA and the COI mtDNA gene sequences. PCR-RFLP diagnostic profiles and PCR with species-specific primers are developed for the studied Scutellonema speciesen_US
dc.description.urihttp://booksandjournals.brillonline.com/content/journals/15685411/15/6
dc.description.urihttp://dx.doi.org/10.1163/15685411-00002714
dc.language.isoenen_US
dc.publisherBrill Academic Publishersen_US
dc.subject28S-rRNAen_US
dc.subjectCOI mtDNAen_US
dc.subjectdiagnosticsen_US
dc.subjectITS rRNAen_US
dc.subjectmorphologyen_US
dc.subjectmorphometricsen_US
dc.subjectmultivariate analysisen_US
dc.subjectPCR-RFLPen_US
dc.subjectPCR with specific primersen_US
dc.subjectScutellonema brachyurusen_US
dc.subjectScutellonema bradysen_US
dc.subjectScutellonema truncatumen_US
dc.subjectSEMen_US
dc.subjectaxonomyen_US
dc.titleMorphological and molecular characterisation and diagnostics of some species of Scutellonema Andrássy, 1958 (Tylenchida: Hoplolaimidae) with a molecular phylogeny of the genusen_US
dc.typeArticleen_US
dc.contributor.researchID10059059 - Tiedt, Louwrens Rasmus


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