Browsing by Subject "Immunofluorescent staining"
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Generation of a set of SA11 expression plasmids for the development of a T7-RNA polymerase-dependent rotavirus reverse genetic system
(North-West University, 2018)Rotavirus (RV) is a member of the family Reoviridae which contains a segmented double-stranded RNA genome comprised of 11 double-stranded RNA genome segments. Rotavirus is still the leading cause of severe diarrhoea worldwide ... -
Local implementation and optimization of rotavirus reverse genetics systems
(North-West University (South Africa)North-West University, 2020)Reverse genetics (RG) is one of the most powerful tools for the study of viral replication, pathogenesis and for the generation of rationally designed vaccine candidates. The main bottleneck in rotavirus (RV) research has ...