Browsing by Subject "Consensus sequence"
Now showing items 1-3 of 3
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Consensus sequence determination and elucidation of the evolutionary history of a rotavirus Wa variant reveal a close relationship to various Wa variants derived from the original Wa strain
(Elsevier, 2013)The consensus nucleotide sequence of a human rotavirus Wa strain, with only a partially known passage history, was determined with sequence-independent amplification and next generation 454 pyrosequencing. This rotavirus ... -
Determination of the whole-genome consensus sequence of the prototype DS-1 rotavirus using sequence-independent genome amplification and 454® pyrosequencing
(Elsevier, 2011)The prototype DS-1 rotavirus strain, is characterised by a short electropherotype and G2P[4] serotype specificity. Following sequence-independent genome amplification and 454® pyrosequencing of genomic cDNA, differences ... -
Local implementation and optimization of rotavirus reverse genetics systems
(North-West University (South Africa)North-West University, 2020)Reverse genetics (RG) is one of the most powerful tools for the study of viral replication, pathogenesis and for the generation of rationally designed vaccine candidates. The main bottleneck in rotavirus (RV) research has ...