Browsing by Subject "Comet assay"
Now showing items 1-10 of 13
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Assessing the DNA methylation status of single cells with the comet assay
(Elsevier, 2010)The comet assay (single cell gel electrophoresis) is a cost-effective, sensitive, and simple technique that is traditionally used for analyzing and quantifying DNA damage in individual cells. The aim of this study was to ... -
Assessing the effects of biochar as an ameliorant for biopesticide polluted soils utilising earthworms
(North-West University (South Africa), 2023)Two plant biopesticides, Bidens pilosa L. and Tagetes minuta L., were investigated for possible effects on earthworms and soil enzymatic activity, compared to the conventional pesticide, Fenamiphos. The efficacy of biochar ... -
DNA damage and repair detected by the comet assay in lymphocytes of African petrol attendants : a pilot study
(North-West University, 2007)Petrol attendants are exposed to petrol volatile organic compounds (VOCs) which may have genotoxic and carcinogenic effects. The single cell gel electrophoresis assay (comet assay) is a method highly sensitive to DNA damage ... -
DNA damage and repair detected by the comet assay in lymphocytes of African petrol attendants: a pilot study
(Oxford Univ Press, 2008)Petrol attendants are exposed to petrol volatile organic compounds (VOCs) which may have genotoxic and carcinogenic effects. The single-cell gel electrophoresis assay (comet assay) is a method highly sensitive to DNA damage ... -
DNA damage and repair in nail technicians caused by occupational exposure to volatile organic compounds
(North-West University, 2010)Objectives: The aim of this study was to determine if exposure to volatile organic compounds can lead to DNA damage and impaired DNA repair capacity. Nail cosmetics is a fast growing industry around the world where employees ... -
Effect of biofumigants on soil microbial communities and ecotoxicology of earthworms (Eisenia andrei)
(Springer, 2016)Biofumigation is considered a good alternative to chemical fumigation because it can control crop pathogens and diseases with lower health and environmental risks than chemical fumigants. Glucosinolates are volatile compounds ... -
The effect of fumigants on earthworms (Eisenia andrei) and soil microbial communities
(North-West University, 2015)Biofumigation is an important crop protection practice that uses a plant’s natural defence mechanisms to control agricultural crop pathogens and diseases. Glucosinolates are volatile compounds found in most Brassica species ... -
Establishing the comet assay to determine the effects of different perturbations on DNA repair capacity
(North-West University, 2011)Single cell gel electrophoresis (SCGE), more commonly known as the Comet assay, is an uncomplicated, affordable and versatile method for investigating DNA damage and repair. Existing comet–assay based methods were modified ... -
Exercise and DNA damage and repair in middle aged men
(North-West University, 2007)Regular physical activity (PA) leads to an increased quality of life by means of certain physiological adaptations. Regular PA is beneficial to the human body and its functionality, including the physiological, biochemical ... -
Hereditary tyrosinemia type 1 metabolites impair DNA excision repair pathways
(Elsevier, 2010)Hereditary tyrosinemia type 1 is an autosomal recessive metabolic disorder, which is caused by a defective fumarylacetoacetate hydrolase enzyme, and consequently metabolites such as succinylacetone and p-hydroxyphenylpyruvate ...