Show simple item record

dc.contributor.authorErasmus, Elardus
dc.contributor.authorMason, Shayne
dc.contributor.authorVan Reenen, Mari
dc.contributor.authorVorster, B. Chris
dc.contributor.authorReinecke, Carolus J.
dc.identifier.citationErasmus, E. et al. 2019. A laboratory approach for characterizing chronic fatigue: what does metabolomics tell us? Metabolomics, 15(12): # 158. []en_US
dc.identifier.issn1573-3890 (Online)
dc.description.abstractIntroduction Manifestations of fatigue range from chronic fatigue up to a severe syndrome and myalgic encephalomyelitis. Fatigue grossly affects the functional status and quality of life of affected individuals, prompting the World Health Organization to recognize it as a chronic non-communicable condition. Objectives Here, we explore the potential of urinary metabolite information to complement clinical criteria of fatigue, providing an avenue towards an objective measure of fatigue in patients presenting with the full spectrum of fatigue levels. Methods The experimental group consisted of 578 chronic fatigue female patients. The measurement design was composed of (1) existing clinical fatigue scales, (2) a hepatic detoxification challenge test, and (3) untargeted proton nuclear magnetic resonance (1H-NMR) procedure to generate metabolomics data. Data analysed via an in-house Matlab script that combines functions from a Statistics and a PLS Toolbox. Results Multivariate analysis of the original 459 profiled 1H-NMR bins for the low (control) and high (patient) fatigue groups indicated complete separation following the detoxification experimental challenge. Important bins identified from the 1H-NMR spectra provided quantitative metabolite information on the detoxification challenge for the fatigue groups. Conclusions Untargeted 1H-NMR metabolomics proved its applicability as a global profiling tool to reveal the impact of toxicological interventions in chronic fatigue patients. No clear potential biomarker emerged from this study, but the quantitative profile of the phase II biotransformation products provide a practical visible effect directing to up-regulation of crucial phase II enzyme systems in the high fatigue group in response to a high xenobiotic-loaden_US
dc.subjectChronic fatigueen_US
dc.subjectDetoxification challenge testen_US
dc.subjectPiper fatigue scaleen_US
dc.subject1H-NMR metabolomicsen_US
dc.subjectPhase II biotransformationen_US
dc.titleA laboratory approach for characterizing chronic fatigue: what does metabolomics tell us?en_US
dc.contributor.researchID10066136 - Erasmus, Elardus
dc.contributor.researchID21487855 - Mason, Shayne William
dc.contributor.researchID12791733 - Van Reenen, Mari
dc.contributor.researchID22713077 - Vorster, Barend Christiaan
dc.contributor.researchID10055037 - Reinecke, Carolus Johannes

Files in this item


There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record