Production of citric acid from the fermentation of pineapple waste by Aspergillus niger
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Date
2019Author
Ayeni, Augustine O.
Sekoai, Patrick T.
Daramola, Michael O.
Taiwo, Olugbenga
Olanrewaju, Omowonuola I.
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Background:
Citric acid, aside its uses as a cleaning agent, has varied applications in the chemical, pharmaceutical, and food industries. A biotechnological fermentation process is one of the easiest ways to satisfy the demands for this useful commodity.
Methods:
The fermentation of pineapple waste by Aspergillus niger for the production of citric acid was investigated in this study. STATISTICA 8 release 7 (Statsoft, Inc. USA) statistical software was used for the design of experiments, evaluation, and optimization of the process using the central composite design (CCD), a response surface methodology approach. Lower-upper limits of the design for the operating parameters were temperature (25-35 oC), fermentation time (35-96 h), pH (3-6), methanol concentration (1-7%) and glucose (15-85 g/L). Twenty-seven duplicated experimental runs were generated for the CCD route.
Results & Conclusion:
The optimal operating conditions were validated at 38 g/L of glucose concentration, 3% (v/v) of methanol, 50 h of fermentation time, pH of 4.3 and temperature of 30 oC which yielded15.51 g/L citric acid. The statistical significance of the model was evaluated using a one-way analysis of variance. The validated predicted response values obtained from the statistical model showed close relationships with the experimental data
URI
http://hdl.handle.net/10394/33344https://benthamopen.com/DOWNLOAD-PDF/TOCENGJ-13-88/
https://doi.org/10.2174/1874123101913010088
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- Faculty of Engineering [1122]