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dc.contributor.authorRashidifard, Milad
dc.contributor.authorFourie, Hendrika
dc.contributor.authorVéronneau, Pierre-Yves
dc.contributor.authorMarais, Mariette
dc.contributor.authorDaneel, Mieke Stefanie
dc.date.accessioned2018-09-28T13:25:52Z
dc.date.available2018-09-28T13:25:52Z
dc.date.issued2018
dc.identifier.citationRashidifard, M. et al. 2018. Genetic diversity and phylogeny of South African Meloidogyne populations using genotyping by sequencing. Scientific reports, 8(1): Article no 13816. [https://doi.org/10.1038/s41598-018-31963-9]en_US
dc.identifier.issn2045-2322 (Online)
dc.identifier.urihttp://hdl.handle.net/10394/31228
dc.identifier.urihttps://doi.org/10.1038/s41598-018-31963-9
dc.identifier.urihttps://www.nature.com/articles/s41598-018-31963-9.pdf
dc.description.abstractMeloidogyne species cause great crop losses worldwide. Although genetic host plant resistance is an effective control strategy to minimize damage caused by Meloidogyne, some resistant genes are ineffective against virulent species such as Meloidogyne enterolobii. Detailed knowledge about the genetic composition of Meloidogyne species is thus essential. This study focused on genotyping-by-sequencing (GBS) and Pool-Seq to elucidate the genetic relation between South African M. enterolobii, M. incognita and M. javanica populations. Hence, 653 common single nucleotide polymorphisms (SNPs) were identified and used to compare these species at genetic level. Allele frequencies of 34 SNPs consistently differed between the three Meloidogyne species studied. Principal component and phylogenetic analyses grouped the M. enterolobii populations in one clade, showing a distant relation to the M. javanica populations. These two species also shared genetic links with the M. incognita populations studied. GBS has been used successfully in this study to identify SNPs that discriminated among the three Meloidogyne species investigated. Alleles, only occurring in the genome of M. enterolobii and located in genes involved in virulence in other animal species (e.g. a serine/threonine phosphatase and zinc finger) have also been identified, accentuating the value of GBS in future studies of this natureen_US
dc.language.isoenen_US
dc.publisherNatureen_US
dc.subjectComparative genomicsen_US
dc.subjectGeneticsen_US
dc.subjectGenomicsen_US
dc.titleGenetic diversity and phylogeny of South African Meloidogyne populations using genotyping by sequencingen_US
dc.typeArticleen_US
dc.contributor.researchID10148620 - Fourie, Hendrika
dc.contributor.researchID27216179 - Rashidifard, Milad


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