Browsing Natural and Agricultural Sciences by Subject "Rotavirus"
Now showing items 1-10 of 10
-
Cloning and evaluation of expression of the open reading frames of a South African G9P[6] rotavirus strain encoding rotavirus structural proteins VP2 and VP6 in bacteria and yeast
(2015)Rotavirus infection causes severe gastroenteritis, affecting all children under the age of five regardless of hygiene or water quality. The currently licensed vaccines succeeded in reducing diarrhoea worldwide, but they ... -
The engineering and optimization of expression of rotavirus–like particles in insect cells using a South African G9P[6] rotavirus strain
(North-West University, 2012)Rotavirus infection causes gastroenteritis, specifically severe gastroenteritis, affecting children younger than five globally, regardless of hygiene and water quality. Current licensed, live, attenuated vaccines do not ... -
Expression of rotavirus proteins and virus-like particles in bacterial and insect cells
(North-West University (South Africa), 2022)Rotavirus infection remains a leading cause of severe gastroenteritis affecting infants and young children. Currently, only live-attenuated (LA) vaccines are licensed and globally administered. These vaccines are not as ... -
Generation of a set of SA11 expression plasmids for the development of a T7-RNA polymerase-dependent rotavirus reverse genetic system
(North-West University, 2018)Rotavirus (RV) is a member of the family Reoviridae which contains a segmented double-stranded RNA genome comprised of 11 double-stranded RNA genome segments. Rotavirus is still the leading cause of severe diarrhoea worldwide ... -
Investigating the feasibility of generating chimeric intergroup rotavirus triple-layered virus-like particles on SA11 double-layered particles
(North-West University (South Africa), 2023)There remains a need for safer and more effective human rotavirus (RV) vaccines, despite the availability of several commercial rotavirus vaccines, especially given that RV is a leading cause of diarrhoea-related deaths ... -
Investigating the importance of co-expressed rotavirus proteins in the development of a selection-free rotavirus reverse genetics system
(2014)Reverse genetics is an innovative molecular biology tool that enables the manipulation of viral genomes at the cDNA level in order to generate particular mutants or artificial viruses. The reverse genetics system for the ... -
Investigating the role of rotavirus GS10(NSP4) and GS6(VP6) in reassortment
(North-West University (South Africa), 2023)Rotaviruses are dsRNA viruses and are the leading causative agent of severe gastroenteritis. All current licenced rotavirus vaccines contain live-attenuated strains and are capable of reassorting their genome segments ... -
Optimization of rotavirus reverse genetics and the rescue of rationally designed rotaviruses
(North-West University (South Africa), 2023)Rotaviruses are the leading cause of diarrhoea related deaths in children under the age of five, despite the availability of vaccines. The currently available rotavirus vaccines are up to 30% less effective in the developing ... -
Preparation of a set of rotavirus SA 11 transcription plasmids for T7- transcript based reverse genetics
(North-West University, 2018)Rotaviruses belong to the Reoviridae family, and rotavirus infection is the biggest contributor to diarrhoea-related death in the world for children under the age of five years. The rotavirus genome consists of 11 ... -
Preparatory investigations for developing a transcript–based rotavirus reverse genetics system
(North-West University, 2012)Reverse genetics systems that are based on either viral transcripts or cDNA genome segments cloned in plasmids have recently been reported for some of the dsRNA viruses of the Reoviridae family, namely African horsesickness ...