dc.contributor.author | Feenstra, Femke | |
dc.contributor.author | Van Rijn, Piet A. | |
dc.contributor.author | Van Gennip, René G.P. | |
dc.contributor.author | Schreuder, Myrte | |
dc.date.accessioned | 2017-05-15T08:23:51Z | |
dc.date.available | 2017-05-15T08:23:51Z | |
dc.date.issued | 2016 | |
dc.identifier.citation | Feenstra, F. et al. 2016. Balance of RNA sequence requirement and NS3/NS3a expression of segment 10 of orbiviruses. Journal of general virology, 97:411-421. [http://dx.doi.org/10.1099/jgv.0.000359] | |
dc.identifier.issn | 0022-1317 | |
dc.identifier.issn | 1465-2099 (Online) | |
dc.identifier.uri | http://hdl.handle.net/10394/23511 | |
dc.identifier.uri | http://dx.doi.org/10.1099/jgv.0.000359 | |
dc.identifier.uri | http://jgv.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.000359 | |
dc.description.abstract | Orbiviruses are insect-transmitted, non-enveloped viruses with a ten-segmented dsRNA
genome of which the bluetongue virus (BTV) is the prototype. Viral non-structural protein
NS3/NS3a is encoded by genome segment 10 (Seg-10), and is involved in different virus
release mechanisms. This protein induces specific release via membrane disruptions and
budding in both insect and mammalian cells, but also the cytopathogenic release that is only
seen in mammalian cells. NS3/NS3a is not essential for virus replication in vitro with BTV
Seg-10 containing RNA elements essential for virus replication, even if protein is not expressed.
Recently, new BTV serotypes with distinct NS3/NS3a sequence and cell tropism have been
identified. Multiple studies have hinted at the importance of Seg-10 in orbivirus replication, but
the exact prerequisites are still unknown. Here, more insight is obtained with regard to the
needs for orbivirus Seg-10 and the balance between protein expression and RNA elements.
Multiple silent mutations in the BTV NS3a ORF destabilized Seg-10, resulting in deletions and
sequences originating from other viral segments being inserted, indicating strong selection at
the level of RNA during replication in mammalian cells in vitro. The NS3a ORFs of other
orbiviruses were successfully exchanged in BTV1 Seg-10, resulting in viable chimeric viruses.
NS3/NS3a proteins in these chimeric viruses were generally functional in mammalian cells, but
not in insect cells. NS3/NS3a of the novel BTV serotypes 25 and 26 affected virus release from
Culicoides cells, which might be one of the reasons for their distinct cell tropism | |
dc.language.iso | en | |
dc.publisher | Microbiology Society | |
dc.title | Balance of RNA sequence requirement and NS3/NS3a expression of segment 10 of orbiviruses | |
dc.type | Article | |
dc.contributor.researchID | 24551287 - Van Rijn, Petrus Antonius | |